06.03.2024
To optimize fertility success, proper handling and storage of semen are crucial factors. INRA 96, a widely trusted semen dilution medium, presents a reliable solution for preparing insemination doses and safeguarding semen quality. Below is a comprehensive guide on how to effectively utilize INRA 96.
A. Preparation of Insemination Doses with INRA 96:
Determining the number of doses.- before semen collection, assess the required number of insemination doses. For each dose needed, withdraw 10 ml of INRA 96 from the bottle and put it in a water bath at +37°C for optimal temperature.
Collection and assessment of semen.- post semen collection, filter it meticulously to eliminate debris and contaminants. Evaluate the semen concentration to ensure precise dilution for achieving an optimal sperm count.
Diluting the semen.- mix the collected semen with INRA 96 to achieve a final concentration of 20 x 10.6 sperm cells per ml. This dilution ensures an ideal sperm count for successful insemination.
Packaging diluted semen.- divide the diluted semen into 10 ml doses. Proper packaging is essential for preserving semen integrity during subsequent storage and transportation.
B. Chilled semen specifics:
Anaerobic storage (4°C).- for storing semen at 4°C, maintain anaerobic conditions to preserve viability. Load 10 ml of diluted semen into a 20 ml syringe without air, sealing it to prevent oxygen exposure.
Aerobic storage (15°C) for poor cooler stallions.- stallions with lower semen quality require storage at 15°C under aerobic conditions. Load 10 ml of diluted semen and 10 ml of air into a 20 ml syringe, ensuring a steady oxygen supply.
C. Additional advice:
Bottle opening caution.- once opened, utilize the contents of the INRA 96 bottle within the day to mitigate the risk of bacterial contamination. The sterilization process that ensures optimal product quality ceases upon bottle opening.
Freezing INRA 96.- if not utilizing the entire medium, split and freeze it in sterile tubes for up to six months. Employ professional-grade equipment for freezing at ≤ -17°C, limiting to a single freezing cycle. Thaw frozen medium in warm water (+37°C) for 30 minutes before use, and avoid exceeding a six-month freezing period.
Syringe Selection.- avoid syringes with black butyl rubber seals on the plunger, as they can be semen-toxic.
By adhering to these guidelines, breeders can maximize the efficacy of INRA 96 for semen preparation and storage, thereby enhancing reproductive outcomes and ensuring the success of breeding programs.
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